One-step polymerized lanthanide-based polystyrene microsphere for sensitive lateral flow immunoassay

作者：Zhenhua Li,Qingyun Liu,Yongfang Li,Wei Yuan,Fuyou Y.Li

摘要：Lateral flow immunoassays(LFIAs)provide a powerful tool for rapid real-time assay of cancer biomarkers,which is vital for cancer detection and treatment follow-up.Lanthanide-based LFIAs is one of the most widely used methods,especially Eu(Ⅲ)chelates,which possess distinctive and attractive characteristics,such as time-resolved fluorescence and large Stokes shift.Herein,we adopted a new onestep mini-emulsion polymerization method to synthesize carboxyl-modified fluorescent microsphere(OS-EuCM),which shows good stability,resistance to non-specific adhesion and uniform particle size distribution compared with traditional microspheres synthesized through the swelling method.Benefiting from the above advantages,OS-EuCM was successfully used in LFIAs to detect tumor marker Alpha-fetoprotein with high sensitivity and selectivity in concentration as high as 320 ng/mL,as well as a detection limit of 0.683 ng/mL This lanthanide-based microsphere holds great potential for rapid pointof-care screening and clinical application.

发文机构：State Key Laboratory of Molecular Engineering of Polymers

关键词：Eu(Ⅲ)chelatePolystyrenemicrosphereMini-emulsionpolymerizationLateralflowimmunoassaysALPHA-FETOPROTEINRareearths

分类号： O657.3[理学—分析化学]R730.4[理学—化学]