Fast genetic mapping in barley:case studies of cuticle mutants using RNA-sequencing

作者：XiaoFeng Li,Chao Li,Qin Zhou,GuoXiong Chen,PengShan Zhao

摘要：Barley(Hordeum vulgare L.)is one of the earliest domesticated crop species and ranked as the fourth largest cereal production worldwide.Forward genetic studies in barley have greatly advanced plant genetics during the last century;however,most genes are identified by the conventional mapping method.Array genotyping and exome-capture sequencing have also been successfully used to target the causal mutation in barley populations,but these techniques are not widely adopted because of associated costs and partly due to the huge genome size of barley.This review summarizes three mapping cases of barley cuticle mutants in our laboratory with the help of RNA-sequencing.The causal mutations have been successfully identified for two of them and the target genes are located in the pericentromeric regions.Detailed information on the mapping-by-sequencing,mapping-and-sequencing,and RNA-sequencing assisted linkage mapping are presented and some limitations and challenges on the mapping assisted by RNA sequencing are also discussed.The alternative and elegant methods presented in this review may greatly accelerate forward genetics of barley mapping,especially for laboratories without large funding.

发文机构：Key Laboratory of Stress Physiology and Ecology in Cold and Arid Regions University of Chinese Academy of Sciences Shanghai Center for Plant Stress Biology Gaolan Station of Agricultural and Ecological Experiment

关键词：BARLEYmapping-by-sequencingRNA-sequencingcuticlepointmutations

分类号： S51[农业科学—作物学]