Comparative transcriptome analysis of abalone Haliotis discus hannai with green and gray egg colors

作者：Yanwei FENG,Bo YUAN,Xu JIANG,Xiangquan LIU,Zan LI,Guohua SUN,Xiaohui XU

摘要：Haliotis discus hannai is an important marine economic species in China.Its egg color was found to be associated with economic traits,which provides a new idea for breeding.However,the molecular mechanism of the egg-color formation has not been reported.Thus,the pigment composition and comparative transcriptome analyses of H.discus hannai with green and gray egg color were conducted using high-performance liquid chromatography(HPLC)and RNA-Seq methods.Results show that individuals with green and gray eggs both possess the fucoxanthin.Lutein existed in gray-egged individuals,but not in green-egged individuals.In transcriptome analysis,272310 unigenes were received from 461162 transcripts with a mean length of 985 bp and N50 of 1524 bp,respectively.A total of 185 unigenes were identifi ed as diff erentially expressed genes(DEGs).The DEGs involved in“fl avin-containing compound metabolic process”,“melanosome”,“glutathione metabolism”,and“cytochrome b6f complex”were likely related to the formation of the egg color.Our results provide foundational information for the functional analysis of egg-color related genes and are benefi cial to the selective breeding of H.discus hannai.

发文机构：School of Agriculture Shandong Provincial Key Laboratory of Restoration for Marine Ecology National Demonstration Center for Experimental Fisheries Science Education Shanghai Engineering Research Center of Aquaculture Shandong Rice Research Institute

关键词：Haliotisdiscushannaieggcolorhigh-performanceliquidchromatographyTRANSCRIPTOMEdifferentiallyexpressedgenes

分类号： O62[理学—有机化学]