Effects of vitamin C deficiency or excess on growth performance, anti-oxidative response and fatty acid composition of juvenile abalone Haliotis discus hannai Ino

作者：LI Xinxin,FENG Xiuni,LUO Kai,MA Shuoli,DENG Junming,ZHANG Wenbing,MAI Kangsen

摘要：A 240-day feeding trial was conducted to investigate the effects of dietary vitamin C on growth performance,anti-oxidative response,and fatty acid composition of juvenile abalone Haliotis discus hannai Ino(initial body weight:0.93±0.00 g).Three semi-purified experimental diets were formulated containing 0.00,94.52,and 9649.58 mg/kg of vitamin C supplied as L-ascorbyl-2-monophosphate.The results show that there was no significant difference in weight gain ratio,daily increment in shell length,and survival rate among the three treatments.Adding dietary vitamin C(9649.58 mg/kg)significantly increased the activities of superoxide dismutase(SOD),glutathine peroxidase(GPX),glitathione-S-transferase(GST),glutathione reductase(GR),alkaline phosphatase(AKP),and lysozyme in viscera(P<0.05).In muscle,activities of phenoloxidase,catalase,SOD,GST,GR,and AKP were increased in the treatment with 9649.58 mg/kg of dietary vitamin C(P<0.05).The highest concentrations of ascorbic acid in viscera and muscle were found in the group with 9649.58 mg/kg of dietary vitamin C(P<0.05).The contents of crude protein and crude lipid in the soft body were significantly increased in the 9649.58 mg/kg group(P<0.05).Dietary vitamin C supplementation significantly decreased the contents of saturated fatty acids(14:0,16:0,and 18:0),and increased the composition of 18:2n-6 and 22:6n-3 in the soft body of abalone(P<0.05).Therefore,although there were no significant effects on the growth performance,dietary vitamin C supplementation improved the anti-oxidation and immune responses,increased specific unsaturated fatty acid(i.e.,16:1,18:1n-7,18:1n-9,18:2n-6 and 22:6n-3),and decreased specific saturated fatty acid(i.e.,14:0,16:0 and 18:0)contents in the soft body of abalone.

发文机构：Key Laboratory of Mariculture（Ministry of Education） Jiaozhou Fisheries Technology Promotion Station College of Animal Science and Technology

关键词：ABALONEascorbicacidanti-oxidativecapabilityfattyacid

分类号： R28[医药卫生—中药学]